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>ASTM E0388-04R23 - Standard Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers
Vydáno: 01.01.2023

ASTM E0388-04R23

Standard Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers

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Označení normy:ASTM E0388-04R23
Vydáno:01.01.2023
Status:Active
Počet stran:3
Sekce:03.06
Označení:fluorescence spectrometers; fluorometer; molecular luminescence; molecular spectroscopy; spectrofluorometer;
ASTM označení:ASTM E388
Nahrazuje:E0388-04R15

This test method covers the testing of the spectral bandwidth and wavelength accuracy of fluorescence spectrometers that use a monochromator for emission wavelength selection and photomultiplier tube detection. The method can be applied to instruments that use multi-element detectors, such as diode arrays, but results must be interpreted carefully. Atomic lines between 250 nm and 1000 nm are used in the method. The difference between the apparent wavelength and the known wavelength for a series of atomic emission lines is used as a test for wavelength accuracy. The apparent width of some of these lines is used as a test for spectral bandwidth.

DESCRIPTION

1.1 This test method covers the testing of the spectral bandwidth and wavelength accuracy of fluorescence spectrometers that use a monochromator for emission wavelength selection and photomultiplier tube detection. This test method can be applied to instruments that use multi-element detectors, such as diode arrays, but results must be interpreted carefully. This test method uses atomic lines between 250 nm and 1000 nm.

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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