Označení normy: | ČSN EN ISO 6579-4 |
Třídící znak: | 560088 |
Počet stran: | 48 |
Vydáno: | 01.07.2025 |
Harmonizace: | Norma není harmonizována |
Katalogové číslo: | 521384 |
Popis
ČSN EN ISO 6579-4
This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression. The method is applicable for: - differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella on-Typhimurium serovar that has the same antigenic formula; - identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium. This document is applicable for the analysis of a pure culture belonging to the genus Salmonella, isolated from: - products intended for human consumption; - products intended for animal feeding; - environmental samples in the area of food and feed production and handling; - samples from the primary production stage. This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health).