This document describes a procedure for the determination of nivalenol (NIV), deoxynivalenol (DON) and its acetyl derivatives (3-acetyl-DON and 15-acetyl-DON), HT-2 and T-2 toxins (HT-2, T-2) and zearalenone (ZEA) in cereals and cereal products by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) after cleanup by solid phase extraction (SPE). The method has been validated with both contaminated and spiked samples of wheat, wheat flour, and wheat crackers. Validation levels for NIV ranged from 27,7¼g/kg to 377,8 ¼g/kg. Validation levels for DON ranged from 233,9¼g/kg to 2420,0 ¼g/kg. Validation levels for 3-acetyl-DON ranged from 18,5¼g/kg to 136,5 ¼g/kg. Validation levels for 15-acetyl-DON ranged from 11,4¼g/kg to 141,8 ¼g/kg. Validation levels for HT-2 ranged from 6,6 ¼g/kg to 133,8 ¼g/kg. Validation levels for T-2 ranged from 2,1 ¼g/kg to 37,6 ¼g/kg. Validation levels for ZEA ranged from 31,6¼g/kg to 229,7 ¼g/kg Laboratory experiences have shown that this method is also applicable to barley and oat flour, and rye based crackers [5], however, this has not been validated in a collaborative study.