21/30422060 DC
This standard 21/30422060 DC BS EN 17641. Foodstuffs. Multimethod for the determination of aflatoxins, deoxynivalenol, fumonisins, ochratoxin A, T-2 toxin, HT-2 toxin and zearalenone by LC-MS/MS is classified in these ICS categories:
- 67.050 General methods of tests and analysis for food products
This document describes an isotope dilution method for the quantitative determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and OTA in infant foods (e.g. infant cereals, milk-based powders), in spices, in dried fruits and in nuts. The method has been validated through an intercollaborative study on different commodity groups: cereals and cereal-based products including food for infant and young children, nuts, spices, dried fruits and milk powder. The ranges of concentrations of each mycotoxin in these naturally contaminated and/or spiked food samples were: - aflatoxin B1: 0,0857 µg/kg - 11,4 µg/kg; - aflatoxin B2: 0,0792 µg/kg - 12,5 µg/kg; - aflatoxin G1: 0,0628 µg/kg - 20,9 µg/kg; - aflatoxin G2: 0,0520 µg/kg - 15,0 µg/kg; - aflatoxin M1: 0,0342 µg/kg - 0,110 µg/kg; - ochratoxin A: 0,448 µg/kg - 17,2 µg/kg; - deoxynivalenol: 45,2 µg/kg - 743 µg/kg; - zearalenone: 9,57 µg/kg - 131 µg/kg; - T-2 toxin: 10,3 µg/kg - 57,9 µg/kg; - HT-2 toxin: 9,50 µg/kg - 81,8 µg/kg; - fumonisin B1: 31,1 µg/kg - 4 262 µg/kg; - fumonisin B2: 44,2 µg/kg - 1 299 µg/kg. The measuring ranges of the method for each mycotoxin/matrix combination are given in Table 7.
