E2719-09R14
Standard Guide for Fluorescence—Instrument Calibration and Qualification
| Označení normy: | E2719-09R14 |
| Vydáno: | 01.05.2014 |
| Status: | Active |
| Počet stran: | 18 |
| Sekce: | 03.06 |
| Označení: | anisotropy; calibration; fluorescence; fluorometer; lifetime; limit of detection; luminescence; qualification; quantum yield; reference materials; spectral correction; spectrometer; spectroscopy; validation; verification; wavelength |
1.1 This guide (1)2 lists the available materials and methods for each type of calibration or correction for fluorescence instruments (spectral emission correction, wavelength accuracy, and so forth) with a general description, the level of quality, precision and accuracy attainable, limitations, and useful references given for each entry.
1.2 The listed materials and methods are intended for the qualification of fluorometers as part of complying with regulatory and other quality assurance/quality control (QA/QC) requirements.
1.3 Precision and accuracy or uncertainty are given at a 1 σ confidence level and are approximated in cases where these values have not been well established.3
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.