BS 684-2.33:1983

This standard BS 684-2.33:1983 Methods of analysis of fats and fatty oils. Other methods is classified in these ICS categories:

• 67.200.10 Animal and vegetable fats and oils

This International Standard specifies a thin-layer chromatographic method for the detection and identification of the following antioxidants in animal and vegetable fats and oils:

• nordihydroguairetic acid (NDGA)
• propylgallate (PG)
• octylgallate (OG)
• dodecylgallate (DG)
• tertiobutylhydroquinone (TBHQ)
• butylhydroxyanisole (tert-butyl-4-methoxyphenol) (BHA)
• α-tocopherol
• butylhydroxytoluene (2,6-di-tert-butyl-4-methylphenol) (BHT)

The antioxidants are listed in the order of their R_f values.

The detection of α-tocopherol is sometimes difficult as the spots of this antioxidant are of a generally diffuse aspect.

The method has limits of detection of 20 mg/kg (ppm) for BHT, and 10 mg/kg (ppm) for the other antioxidants. It should be noted that BHT is not completely isolated by this method.

Describes thin-layer chromatography for nordihydroguairetic, propylgallate, octylgallate, dodecylgallate, tertiobutylhydroquinone, butylhydroxyanisole, α-tocopherol and butylhydroxytoluene.