BS EN 17252:2020

This standard BS EN 17252:2020 Foodstuffs. Determination of phomopsin A in lupin seeds and lupin derived products by HPLC-MS/MS is classified in these ICS categories:

• 67.060 Cereals, pulses and derived products

This document specifies a procedure for the determination of phomopsin A in lupin seeds and lupin-derived products based on liquid chromatography with tandem mass spectrometry (LC-MS/MS). Several phomopsins exist, i.e. phomopsin A, B, C and D, but the method only deals with the quantitative measurement of phomopsin A due to lack of commercially available analytical reference standards for the other phomopsins.

The method has been validated for phomopsin A in naturally contaminated lupin seeds, lupin flour and crisp bread at levels ranging from approximately 5 µg/kg to 60 µg/kg.