BS EN ISO 15216-1:2017+A1:2021

This standard BS EN ISO 15216-1:2017+A1:2021 Microbiology of the food chain. Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR is classified in these ICS categories:

• 07.100.30 Food microbiology

This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.