BS EN ISO 20837:2006

This standard BS EN ISO 20837:2006 Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Requirements for sample preparation for qualitative detection is classified in these ICS categories:

• 07.100.30 Food microbiology

This International Standard provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.

It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.

This International Standard has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.