This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or cold smoked.

This method is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature.

This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.