This document specifies a method for the determination of the content of undissolved substances, referred to as total contamination, in neat fatty acid methyl esters (FAME). The working range is from 5 mg/kg to 27 mg/kg and it was established in an interlaboratory study by applying EN ISO 4259-1 [1]. This document in general applies to products having a kinematic viscosity not exceeding 8 mm2/s at 20 °C, or 5 mm2/s at 40 °C, e.g. FAME as specified in EN 14214 [2]. NOTE For the purposes of this document, the term % (V/V) is used to represent the volume fraction, Æ, of a material. WARNING Use of this test method may involve hazardous materials, operations and equipment. This method does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this method to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.