This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. It is applicable to the following: microbial plant biostimulants. NOTE 1 The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1]. NOTE 2 For confirmation, it is possible to use PCR tests; in this case the laboratory must validate the procedure and data generated.