This document specifies methods for the determination of five selected estrogens in whole water samples listed in Table 1. The methods are based on solid-phase extraction (SPE disk and/or cartridge) followed by liquid or gas chromatography-mass spectrometry detection (tandem mass spectrometry and/or High Resolution Mass Spectrometry). Depending on the sample preparation chosen, it is applicable to the analysis of selected estrogens in drinking water, groundwater and surface water containing suspended particulate matter (SPM) up to 500 mg/l, DOC content up to 14 mg/l (whole water samples). The lower application range defined as verified limit of quantification can vary depending on the methods the sensitivity of the equipment used and the matrix of the sample. The range is 0,006 ng/l to 1 ng/l for EE2 and 0,038 ng/l to 1 ng/l for the other estrogens in drinking water, ground water and surface water. The upper limit of the working range is approximately tens ng/l. For application that targets the measurements of very low level of concentrations (between the lowest LOQ and 0,1 ng/l) every single step of the methods become critical and imply to fix some additional requirements. The method can be used to determine further estrogens or hormones in other types of water e.g. treated wastewater if accuracy has been tested and verified for each case as well as storage conditions of both samples and reference solutions have been validated.